ToxicologyUnder typical in vitro cell culture conditions, oxygen consumption rate is a direct measurement of mitochondrial respiration. The extracellular acidification rate is dominated by lactic acid production formed during glycolytic energy metabolism. The study of the adverse effects of chemical and physical or biological agents on living organisms and the ecosystem imply the generation of information about time-dependent and recovery effects, calculation of dose-responses and dynamic IC50 values.
The Bionas® analyzing system enables the investigation of toxic effects in areas like:
- Developmental toxicity: The analysis of chemicals which cause functional and morphological effects on a developing organism.
- Immunotoxicity: Investigation of immune modulating effects of compounds on cells of the immune system.
- General toxicity: Effects of toxic compounds on cell lines and primary cells of any origin.
- Cardiotoxicity: Cardiomyocytes are used for pharmacological and toxicological assays with a focus on long-term measurements.
- Hepatotoxicity: Primary human and rat hepatocytes as well as hepatocytic cell lines can be investigated to predict liver toxicity of test compounds.
- Environmental toxicity: Investigation of environmental toxins and contaminants like solvents and heavy metals, which cause problems by disrupting the functional properties in the living organism.
- Nanotoxicity: The Bionas® analyzing system offers an alternative approach to animal testing to assess the toxicological risks of soluble chemicals and insoluble micro and nano particles.
App Note 001: Evaluating agarose embeddes suspension cells (lymphocytes) using the Bionas® 2500 analyzing system
App Note 002: Prediction of hepatotoxic effects in hepatocytes from different sources using the Bionas® 2500 analyzing system
App Note 003: temporal analysis of the pharmacodynamic effects of cycloheximide using the Bionas® 2500 analyzing system